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To assess whether Cas12f delivery via EVs requires MIM, we attenuated protrusion formation by introducing a five-lysine-to-alanine (5KA) mutation in the MIM I-BAR domain 54 , which was previously shown to impair EV generation 40

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PMID 17175815

Tissue PS and IS contents are significantly increased in the renal cortex of kidney failure mice shown by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) 30

The concentrations and yields of GSH based on l-cysteine for these engineered strains are shown in Fig